Principle
WGBS combines bisulfite conversion with sequencing to estimate DNA methylation from converted bases. It supports genome-scale comparisons of methylation associated with development, disease or experimental treatment.
Applications
- Investigate methylation during development and cell-state changes
- Compare methylation sites and regions before and after treatment
- Integrate transcriptomics to study methylation-expression relationships
Method features
- Single-base resolution within regions actually covered
- Supports genome-scale methylation research
- Compares methylation at both site and regional levels
Workflow
- 01
Extract DNA and assess quality
- 02
Perform bisulfite conversion and appropriate library preparation
- 03
Sequence and assess conversion and coverage
- 04
Quantify methylation and annotate differential sites and regions
This is a workflow overview. Experimental and analytical details depend on sample conditions and research objectives.